- Automated Droplet Generator 1864101 User Manual Software
- Automated Droplet Generator 1864101 User Manual Free
- Automated Droplet Generator 1864101 User Manual 2016
- Automated Droplet Generator 1864101 User Manual Download
Oct 31, 2014 The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The AutoDG Instrument eliminates user-to-user variability, allowing you to fully.
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Instruction
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- The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates.
- 14 Automated Droplet Generator. Using the Automated Droplet Generator. The cooling block should be placed in a –20°C freezer for at least 2 hours before configuring a run on the AutoDG Instrument and inserting the Droplet Plate assembly into the instrument.
Manual | 13
12 | Automated Droplet Generator
Using the Automated Droplet Generator
2.2 Starting a Run from Idle Mode on the AutoDG
8. To load the 96-well PCR plate containing your prepared ddPCR reactions into the front row of the instrument:
a. The sample plate can be sealed with a PX1
™
Plate Sealer and heat-sealing foil in advance of loading
into the AutoDG Instrument. Each well should contain 22 µl of your prepared ddPCR reaction. Please
see the supermix product insert for more detailed information on ddPCR reaction setup.
see the supermix product insert for more detailed information on ddPCR reaction setup.
b. Place the plate into the front left plate holder, labeled on the screen as Sample Plate. The holder is
keyed for proper orientation and contains plate clips to support sealed plates.
c. The light on the Sample Plate holder will change from yellow to green when the plate is inserted
correctly. If the light remains yellow, try repositioning the plate in a different orientation.
d. As the light turns green on the deck, the corresponding icon on the touch screen will go from blinking
yellow to solid green, and Ready will be displayed.
Please note that a sample plate is required for every run, regardless of the number of columns selected.
Fig. 9. Pipet tip boxes have been correctly loaded. The Ready
status appears on the Pipet Tip icons on the screen.
status appears on the Pipet Tip icons on the screen.
Fig. 10. Sample plate has been correctly loaded. The Ready
status appears on the Sample Plate icon on the screen.
status appears on the Sample Plate icon on the screen.
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Automated Droplet Generator 1864101 User Manual Software
Instruction
Manual | 17
16 | Automated Droplet Generator
Using the Automated Droplet Generator
2.2 Starting a Run from Idle Mode on the AutoDG
12. Once you have confirmed the plate setup, touch the Start Run button to begin droplet generation. The door
will automatically close at the beginning of the run and must remain closed during the run. Opening the door
before the Droplets Ready message appears will cause the instrument to terminate the run.
before the Droplets Ready message appears will cause the instrument to terminate the run.
If the selected columns on the confirmation screen do not match the location of samples in your sample
plate, touch Back to change. Touch the sample plate to bring up the Configure Sample Plate window and
change your selection.
plate, touch Back to change. Touch the sample plate to bring up the Configure Sample Plate window and
change your selection.
13. After a brief initialization, the Automated Droplet Generator will display a countdown timer on the screen
with time remaining until the plate of droplets is ready. No additional action is needed until the plate is
ready. The initialization can take 1–5 min, depending on whether the oil type has been changed or not
(see Section 2.4).
ready. The initialization can take 1–5 min, depending on whether the oil type has been changed or not
(see Section 2.4).
Fig. 16. The AutoDG door automatically closes at the start of each
run and must remain closed until droplet generation is complete.
run and must remain closed until droplet generation is complete.
Automated Droplet Generator 1864101 User Manual Free
Fig. 17. A, initializing display at the beginning of a run; B, countdown timer display indicates the time remaining until droplet
generation is complete and the Droplet Plate can be removed.
generation is complete and the Droplet Plate can be removed.
Automated Droplet Generator 1864101 User Manual 2016
A B
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